Hydrogen ion currents in rat alveolar epithelial cells
نویسندگان
چکیده
منابع مشابه
Na(+)-H+ antiport detected through hydrogen ion currents in rat alveolar epithelial cells and human neutrophils
Voltage-activated H(+)-selective currents were studied in cultured adult rat alveolar epithelial cells and in human neutrophils using the whole-cell configuration of the patch-clamp technique. The H+ conductance, gH, although highly selective for protons, was modulated by monovalent cations. In Na+ and to a smaller extent in Li+ solutions, H+ currents were depressed substantially and the voltag...
متن کاملThe voltage-activated hydrogen ion conductance in rat alveolar epithelial cells is determined by the pH gradient
Voltage-activated H+ currents were studied in rat alveolar epithelial cells using tight-seal whole-cell voltage clamp recording and highly buffered, EGTA-containing solutions. Under these conditions, the tail current reversal potential, Vrev, was close to the Nernst potential, EH, varying 52 mV/U pH over four delta pH units (delta pH = pHo - pHi). This result indicates that H+ channels are extr...
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Inactivation of K+ channels responsible for delayed rectification in rat type II alveolar epithelial cells was studied in Ringer, 160 mM K-Ringer, and 20 mM Ca-Ringer. Inactivation is slower and less complete when the extracellular K+ concentration is increased from 4.5 to 160 mM. Inactivation is faster and more complete when the extracellular Ca2+ concentration is increased from 2 to 20 mM. Se...
متن کاملCurrents in Human Neutrophils, Rat Alveolar Epithelial Cells, and Mammalian Phagocytes
H 1 currents in human neutrophils, rat alveolar epithelial cells, and several mammalian phagocyte cell lines were studied using whole-cell and excised-patch tight-seal voltage clamp techniques at temperatures between 6 and 42 8 C. Effects of temperature on gating kinetics were distinguished from effects on the H 1 current amplitude. The activation and deactivation of H 1 currents were both high...
متن کاملAlveolar Epithelial Ion and Fluid Transport Na transport proteins are expressed by rat alveolar epithelial type I cells
Borok, Zea, Janice M. Liebler, Richard L. Lubman, Martha J. Foster, Beiyun Zhou, Xian Li, Stephanie M. Zabski, Kwang-Jin Kim, and Edward D. Crandall. Na transport proteins are expressed by rat alveolar epithelial type I cells. Am J Physiol Lung Cell Mol Physiol 282: L599–L608, 2002; 10.1152/ajplung.00130.2000.—Despite a presumptive role for type I (AT1) cells in alveolar epithelial transport, s...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1991
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(91)82158-0